mouse anti elav 9f8a9  (Developmental Studies Hybridoma Bank)

Journal: Microbiology Spectrum

Article Title: Wolbachia infection confers post-translational modification of glutamic acid decarboxylase and other proteins in D. melanogaster

doi: 10.1128/spectrum.02465-24

Figure Lengend Snippet: GABA levels do not change overall upon Wolbachia infection in D. melanogaster . ( A ) Comparison of GABA content in Wolbachia -infected and uninfected head lysates (normalized to the protein levels). N = 5 replicates of 100 heads each; error bars represent SEM. ( B ) Representative images of GABA immunostaining of w 1118 brains with and without Wolbachia . Brains were also stained with an antibody against ELAV to visualize neurons. Images are Z-projections of approximately 50 µM stacks. Scale bar = 25 µM. ( C ) Quantification of GABA staining in immunostained brains, separated by sex and mating status. GABA intensity was normalized against ELAV staining for each brain. N ≥ 8 for each group, * P < 0.05.

Article Snippet: All samples were stained with a polyclonal rabbit antibody against GABA (Sigma #A2052; 1:1000) and a monoclonal mouse antibody against ELAV (DSHB Hybridoma Product Elav-9F8A9; 1:25).

Techniques: Infection, Comparison, Immunostaining, Staining